Remember that we are adjusting the phase along f2, so do not worry if the phase of the peaks along the indirect (vertical) dimension ( f1) is not correct. The goal ( 1d) will be to make all the signals of the area of interest (highlighted with the green circle in the picture above) positive (note that in this example, positive signals are red whereas negative are blue). Then, take a look at the signal, or the group of signals ( 1a), which appear in a corner of the spectrum (for example, the signals highlighted with a green circle in the capture above) select 'manual correction' ( 1b) from the drop down menu on the 'Phase Correction' toolbar button and adjust the zero order (PH0) phase parameter along f2 (by dragging the mouse up or down over the blue rectangle in the 'Phase Correction dialog box' whilst holding the left mouse button ( 1c)).

Once you have opened the spectrum 'zoom in' the area of interest (in this case, we only need to worry about the left half of the spectrum):ġ. We will show how to correct the phase of a gNHSQC experiment (see figure below).

In this tutorial, we will explain how to phase the spectrum in real time, directly on the 2D spectrum.

Mestrenova 2d nmr